Regulatory Properties of Perchlorate-extracted Succinate Dehydrogenase
نویسندگان
چکیده
منابع مشابه
Molecular properties of succinate dehydrogenase isolated from Micrococcus luteus (lysodeikticus).
Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polypeptide possessed a covalently bound flavin prosthetic group and appeared to be ...
متن کاملStudies on Succinate Dehydrogenase
1. Succinate dehydrogenase flavocoenzyme (“SD-flavin”), previously shown to be an SOLsubstituted riboflavin derivative containing a tertiary nitrogen homoconjugated to the flavin nucleus, was subjected to further hydrolysis and to reduction under acid conditions. Both conditions resulted in the liberation of 1 mole of histidine per mole of flavin. This proves histidine to be the covalent link b...
متن کاملSingle motoneuron succinate dehydrogenase activity.
We have developed a quantitative histochemical assay for measurement of succinate dehydrogenase (SDH) activity in single motoneurons. A computer image processing system was used to quantify the histochemical enzyme reaction product and to follow the time course of the reaction. The optimal concentration for each of the ingredients of the incubation medium for the SDH reaction was determined and...
متن کاملInactivation of succinate dehydrogenase by 3-nitropropionate.
This paper examines proposals in the literature that 3-nitropropionate is a suicide inactivator of succinate dehydrogenase and that it acts by nucleophilic addition to N-5 of the covalently bound flavin component of the enzyme. With purified, soluble preparations of the enzyme, the inhibition developed slowly, and nearly complete inactivation occurred with a stoichiometric amount of 3-nitroprop...
متن کاملThe synthesis of succinate-dehydrogenase riboflavin.
Singer and Kearney"], in their early studies, recognized that the flavocoenzyme of mitochondria1 succinate dehydrogenase ("SD-flavin") [**I was a derivative of flavin-adenine-dinucleotide which was covalently bound to the protein. In cooperation with Dr. Singer's team [***I, we have attempted to elucidate the structure of this coenzyme. In the first preliminary communication I21, we concluded f...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43041-x